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Mouse L-Selectin/SELL ELISA Kit

时间:2016-10-28 07:30:26来源:本站 作者:admin 点击:191次


【项目简介】
L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naive and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠L-Selectin抗体,当加入标准品和待测样本时,标准品和待测样本中的小鼠L-Selectin会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗小鼠L-Selectin抗体可与被酶标板捕获的小鼠L-Selectin结合,形成“包被抗小鼠L-Selectin抗体+小鼠L-Selectin+抗小鼠L-Selectin抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,小鼠L-Selectin浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠L-Selectin的浓度。


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