【项目简介】
CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a homeostatic chemokine that signals through CCR7 or CXCR3. CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) where it promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation. Its upregulation during chronic inflammation or tissue damage promotes fibrosis, inflammatory cytokine production, and neuropathic pain. The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CCL21抗体，当加入标准品和待测样本时，重组或天然的人CCL21会和该抗体结合。洗板后，游离的其他成分被洗去，加入生物素化的抗人CCL21抗体可与被酶标板捕获的人CCL21结合。再次洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的链霉亲和素，生物素和链霉亲和素特异性结合，形成“包被抗人CCL21抗体+人CCL21+生物素化抗人CCL21抗体+链霉亲和素-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人CCL21浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人CCL21的浓度。