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Human Eotaxin-3 ELISA Kit

时间:2016-10-27 03:58:58来源:本站 作者:admin 点击:222次


【项目简介】
Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人Eotaxin-3抗体,当加入标准品和待测样本时,重组或天然的人Eotaxin-3会和该抗体结合。洗板后,游离的其他成分被洗去,加入生物素化的抗人Eotaxin-3抗体可与被酶标板捕获的人Eotaxin-3结合。再次洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的链霉亲和素,生物素和链霉亲和素特异性结合,形成“包被抗人Eotaxin-3抗体+人Eotaxin-3+生物素化抗人Eotaxin-3抗体+链霉亲和素-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人Eotaxin-3浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人Eotaxin-3的浓度。


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