【项目简介】
RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily, designated TNFRSF19-like (TNFRSF19L). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes 430 amino acids (aa), including a 25 aa signal peptide, a 137 aa extracellular domain containing a TNF receptor cysteine-rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region that lacks a death domain. Within the ECD, human RELT shares 78%, 75%, 75%, 78% and 72% aa sequence homology with mouse, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40. Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested. Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse. RELL1 and 2 can interact with and are coexpressed with RELT, and are thought to modulate its signaling. Intracellularly, RELT has been shown to bind the adaptor protein TRAF-1 and activate the NF‑ kappa B pathway, the phospholipid scramblase PLSCR1, and oxidative stress responsive proteins OSR1.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人RELT抗体，当加入标准品和待测样本时，标准品和待测样本中的人RELT会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人RELT抗体可与被酶标板捕获的人RELT结合，形成“包被抗人RELT抗体+人RELT+抗人RELT抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人RELT浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人RELT的浓度。