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Mouse FGFR3/CD333 ELISA Kit

时间:2016-10-29 11:26来源:未知 作者:admin 点击:
Mouse FGFR3/CD333 ELISA Kit 96T 3500元(货号EM95-96)


【项目简介】
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform. Only the alpha  isoform has been identified for FGF R3 and FGF R4. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠FGF R3抗体,当加入标准品和待测样本时,标准品和待测样本中的小鼠FGF R3会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗小鼠FGF R3抗体可与被酶标板捕获的小鼠FGF R3结合,形成“包被抗小鼠FGF R3抗体+小鼠FGF R3+抗小鼠FGF R3抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,小鼠FGF R3浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠FGF R3的浓度。


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