联系我们
  • 电话: 010-57346902
  • 邮箱:sale@bio-kits.cn
  • 网站: www.bio-kits.cn
  • 地址: 北京市昌平科技园区凉水河路1号院

Mouse MEP1A ELISA Kit

时间:2016-10-29 11:32来源:未知 作者:admin 点击:
Mouse MEP1A ELISA Kit 96T 3500元(货号EM96-96)


【项目简介】
Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases. Both subunits form disulfide‑linked homo‑ or hetero‑oligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post‑translational processing and subsequently cellular location, and substrate and peptide bond specificity. The 760 amino acid sequence of mouse meprin alpha subunit precursor consists of a signal peptide (residues 1‑33), a pro region (residues 34‑77), and a mature chain (residues 78‑760) containing the following domains, catalytic (residues 78‑275), MAM (residues 276‑445), MATH (residues 447‑607), EGF‑like (residues 684‑724), transmembrane (residues 727‑754), and cytoplasmic (residues 755‑760). The pro enzyme terminating at residue 615 was expressed and the secreted protein purified from conditioned medium. The molecular masses of recombinant mouse MEP1A are similar to those observed for the alpha subunit of rat Meprin A.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠MEP1A抗体,当加入标准品和待测样本时,标准品和待测样本中的小鼠MEP1A会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗小鼠MEP1A抗体可与被酶标板捕获的小鼠MEP1A结合,形成“包被抗小鼠MEP1A抗体+小鼠MEP1A+抗小鼠MEP1A抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,小鼠MEP1A浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠MEP1A的浓度。


------分隔线----------------------------