【项目简介】
CD34 is a 100-105 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34+ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. There is one 90 kDa splice variant that shows a four aa substitution for aa 322-382. Mouse CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat CD34, respectively.  
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠CD34抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠CD34会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠CD34抗体可与被酶标板捕获的小鼠CD34结合，形成“包被抗小鼠CD34抗体+小鼠CD34+抗小鼠CD34抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠CD34浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠CD34的浓度。