【项目简介】
Mouse Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors. It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages. The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain. Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection. It enhances entry of cationic inhibitors into macrophages. It inhibits both insulin receptor autophosphorylation and undesirable calcification.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠Fetuin-A抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠Fetuin-A会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠Fetuin-A抗体可与被酶标板捕获的小鼠Fetuin-A结合，形成“包被抗小鼠Fetuin-A抗体+小鼠Fetuin-A+抗小鼠Fetuin-A抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠Fetuin-A浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠Fetuin-A的浓度。