【项目简介】
Mouse IL-13 receptor alpha one (IL‑13 R alpha 1), previously called NR4, IL-13 R alpha, and IL13R alpha ', is a member of the hemopoietin receptor family that was cloned on the basis of its conserved WSXWS motif. Mouse IL‑13 R alpha 1 encodes a 424 amino acid (aa) residue precursor type I membrane protein with a 26 aa residue signal peptide, a 314 aa residue extracellular domain (containing an immunoglobulin-like domain and a typical hemopoietin receptor domain), a 24 aa residue transmembrane region and a 60 aa residue cytoplasmic tail. Human IL‑13 R alpha 1, with 76% amino acid sequence identity to mouse IL‑13 R alpha 1, has also been cloned. In addition, a second human IL‑13 binding protein, designated IL‑13 R alpha 2 and previously known as IL‑13 R beta, IL‑13 R and IL‑13 R alpha, has been cloned from the Caki‑1 human renal carcinoma cell line. Murine IL‑13 R alpha 2 has been cloned from a mouse thymus cDNA library. The amino terminal 27 amino acid residues of the human and mouse IL‑13 R alpha 2 is nearly identical to that of a soluble mouse IL ‑3 binding proteinpurified from mouse serum and urine.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠IL-13RA1抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠IL-13RA1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠IL-13RA1抗体可与被酶标板捕获的小鼠IL-13RA1结合，形成“包被抗小鼠IL-13RA1抗体+小鼠IL-13RA1+抗小鼠IL-13RA1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠IL-13RA1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠IL-13RA1的浓度。