【项目简介】
Interferon‑alpha/beta receptor 1 (IFN‑ alpha / beta  R1), also known as IFNAR1, is a 100‑130 kDa member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions. IFN‑ alpha/beta  R1, in association with IFN‑ alpha / beta R2, is required for propagating anti‑microbial signal transduction triggered by the type 1 interferons such as IFN‑ alpha and IFN‑ beta. Mature mouse IFN‑ alpha / beta  R1 consists of a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain. The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, mouse IFN‑ alpha / beta  R1 shares 47% and 68% aa identity with human and rat IFN‑ alpha / beta  R1, respectively. IFN‑ alpha/beta  R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN‑ alpha/beta  R2. Ligands preferentially associate with IFN‑ alpha / beta  R2, and this complex subsequently forms a stable ternary assembly with IFN‑alpha/beta  R1. IFN‑alpha/beta ‑R1 also associates with IFN‑ gamma  R2 even in the absence of IFN‑ gamma stimulation. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠IFNAR1抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠IFNAR1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠IFNAR1抗体可与被酶标板捕获的小鼠IFNAR1结合，形成“包被抗小鼠IFNAR1抗体+小鼠IFNAR1+抗小鼠IFNAR1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠IFNAR1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠IFNAR1的浓度。