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Mouse Endoglin/CD105/ENG ELISA Kit

时间:2016-10-28 11:03:47来源:本站 作者:admin 点击:322次


【项目简介】
Endoglin, also known as CD105, is a type I  homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠Endoglin抗体,当加入标准品和待测样本时,标准品和待测样本中的小鼠Endoglin会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗小鼠Endoglin抗体可与被酶标板捕获的小鼠Endoglin结合,形成“包被抗小鼠Endoglin抗体+小鼠Endoglin+抗小鼠Endoglin抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,小鼠Endoglin浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠Endoglin的浓度。


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