【项目简介】
CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth. Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities. It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA‑derived bone marrow stromal cell lines. CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠BST1抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠BST1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠BST1抗体可与被酶标板捕获的小鼠BST1结合，形成“包被抗小鼠BST1抗体+小鼠BST1+抗小鼠BST1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠BST1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠BST1的浓度。