【项目简介】
Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The deduced amino acid sequence of mouse CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 108), and a mature chain (residues 109 to 415). The purified rmCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠CPB1抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠CPB1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠CPB1抗体可与被酶标板捕获的小鼠CPB1结合，形成“包被抗小鼠CPB1抗体+小鼠CPB1+抗小鼠CPB1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠CPB1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠CPB1的浓度。