【项目简介】
TIM‑1 (T cell‑immunoglobulin‑mucin; also KIM‑1 and Tapr) is a 70 ‑ 80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2‑cell‑mediated immunity. In mouse, there are eight known TIM genes (# 1 ‑ 8) vs. only three genes in human (# 1, 3 & 4). Mouse TIM‑1 and ‑2 are counterparts of human TIM‑1, while mouse TIM‑5 through TIM‑8 have no human counterparts. Mouse TIM‑1 (isoform 2) is synthesized as a 282 amino acid (aa) precursor that contains a 21 aa signal sequence, a 193 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain. The ECD contains one V‑type Ig‑like domain and a mucin region characterized by multiple T‑S‑P motifs. The mucin region undergoes extensive O‑linked glycosylation. The mouse TIM‑1 gene is highly polymorphic and, based on rat, may undergo alternate splicing. One isoform (termed isoform 1) possesses a 23 aa insertion after Pro182 (GenBank # NP_599099). Another splice variant (of isoform 1) shows a 15 aa deletion in the mucin region of the ECD. This difference is associated with a decreased susceptibility to asthma. In human, TIM‑1 is known to circulate as a soluble form that arises from cleavage by an undefined MMP, releasing an 85 ‑ 90 kDa soluble molecule. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠TIM-1抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠TIM-1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠TIM-1抗体可与被酶标板捕获的小鼠TIM-1结合，形成“包被抗小鼠TIM-1抗体+小鼠TIM-1+抗小鼠TIM-1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠TIM-1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠TIM-1的浓度。