联系我们
  • 电话: 010-57346902
  • 邮箱:sale@bio-kits.cn
  • 网站: www.bio-kits.cn
  • 地址: 北京市昌平科技园区凉水河路1号院

Human BMP-7 ELISA Kit

时间:2019-02-12 12:55来源:未知 作者:admin 点击:
Human BMP-7 ELISA Kit 96T 2500元(货号EH302-96)
【项目简介】
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人BMP-7抗体,当加入标准品和待测样本时,重组或天然的人BMP-7会和该抗体结合。洗板后,游离的其他成分被洗去,加入生物素化的抗人BMP-7抗体可与被酶标板捕获的人BMP-7结合。再次洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的链霉亲和素,生物素和链霉亲和素特异性结合,形成“包被抗人BMP-7抗体+人BMP-7+生物素化抗人BMP-7抗体+链霉亲和素-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人BMP-7浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人BMP-7的浓度。



------分隔线----------------------------