【项目简介】
The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation, palate development, branching morphogenesis and directional outgrowth of lung buds, formation of the otic vesicle and chochlea, adipogenesis, and the development of prostate, mammary, lacrimal, and submandibular salivary glands. FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology. 

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人FGF10抗体，当加入标准品和待测样本时，标准品和待测样本中的人FGF10会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人FGF10抗体可与被酶标板捕获的人FGF10结合，形成“包被抗人FGF10抗体+人FGF10+抗人FGF10抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人FGF10浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人FGF10的浓度。