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Human NOV/CCN3 ELISA Kit

时间:2016-10-28 03:41:15来源:本站 作者:admin 点击:341次


【项目简介】
Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development , and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人NOV抗体,当加入标准品和待测样本时,标准品和待测样本中的人NOV会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗人NOV抗体可与被酶标板捕获的人NOV结合,形成“包被抗人NOV抗体+人NOV+抗人NOV抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人NOV浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人NOV的浓度。


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