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Rat PCSK9 ELISA Kit

时间:2016-10-29 11:37来源:未知 作者:admin 点击:
Rat PCSK9 ELISA Kit 96T 3500元(货号ER22-96)


【项目简介】
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠PCSK9抗体,当加入标准品和待测样本时,标准品和待测样本中的大鼠PCSK9会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗大鼠PCSK9抗体可与被酶标板捕获的大鼠PCSK9结合,形成“包被抗大鼠PCSK9抗体+大鼠PCSK9+抗大鼠PCSK9抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠PCSK9浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠PCSK9的浓度。


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