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Rat CD86/B7-2 ELISA Kit

时间:2016-10-29 11:52来源:未知 作者:admin 点击:
Rat CD86/B7-2 ELISA Kit 96T 3500元(货号ER24-96)


【项目简介】
B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. Mature rat B7-2 consists of a 222 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 19 aa transmembrane segment, and a 46 aa cytoplasmic tail. Within the ECD, rat B7-2 shares 59% and 81% aa sequence identity with human and mouse B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes, as well as on vascular endothelial cells. B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co‑stimulation. B7‑1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation. CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function. B7-2 is expressed earlier than B7-1 following APC activation, and both proteins bind with higher affinity to CTLA-4 than to CD28. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠B7-2抗体,当加入标准品和待测样本时,标准品和待测样本中的大鼠B7-2会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗大鼠B7-2抗体可与被酶标板捕获的大鼠B7-2结合,形成“包被抗大鼠B7-2抗体+大鼠B7-2+抗大鼠B7-2抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠B7-2浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠B7-2的浓度。


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