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Rat IL-1R1/CD121a ELISA Kit

时间:2016-10-29 11:59来源:未知 作者:admin 点击:
Rat IL-1R1/CD121a ELISA Kit 96T 3500元(货号ER25-96)


【项目简介】
The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL-1 RI is an 80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88, to initiate signaling in the NF kappa B pathway. Rat IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Rat IL-1 RI shares 83%, 65%, 60%, 60% and 54% aa identity with mouse, human, canine, equine and bovine IL-1 RI, respectively. Two additional splice isoforms of rat IL-1 RI have been described. One has an N-terminus that is extended by 14 amino acids, but appears to have equivalent function. The other lacks the TM sequence and is secreted as an ~82 kDa protein that antagonizes the effect of IL-1 beta. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠IL-1R1抗体,当加入标准品和待测样本时,标准品和待测样本中的大鼠IL-1R1会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗大鼠IL-1R1抗体可与被酶标板捕获的大鼠IL-1R1结合,形成“包被抗大鼠IL-1R1抗体+大鼠IL-1R1+抗大鼠IL-1R1抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠IL-1R1浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠IL-1R1的浓度。


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