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Rat Tissue Factor/CD142 ELISA Kit

时间:2016-10-29 12:05来源:未知 作者:admin 点击:
Rat Tissue Factor/CD142 ELISA Kit 96T 3500元(货号ER26-96)


【项目简介】
Tissue Factor (TF), also known as Thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of coagulation factor VII, which is synthesized in the liver and circulated in the plasma. Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1 to 32) and the mature chain (residues 33 to 295), which includes a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 295).
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠Tissue Factor 抗体,当加入标准品和待测样本时,标准品和待测样本中的大鼠Tissue Factor 会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗大鼠Tissue Factor 抗体可与被酶标板捕获的大鼠Tissue Factor 结合,形成“包被抗大鼠Tissue Factor 抗体+大鼠Tissue Factor +抗大鼠Tissue Factor 抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠Tissue Factor 浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠Tissue Factor 的浓度。


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