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Rat E-Selectin ELISA Kit

时间:2016-10-29 12:27来源:未知 作者:admin 点击:
Rat E-Selectin ELISA Kit 96T 3500元(货号ER29-96)


【项目简介】
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠E-Selectin抗体,当加入标准品和待测样本时,标准品和待测样本中的大鼠E-Selectin会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗大鼠E-Selectin抗体可与被酶标板捕获的大鼠E-Selectin结合,形成“包被抗大鼠E-Selectin抗体+大鼠E-Selectin+抗大鼠E-Selectin抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠E-Selectin浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠E-Selectin的浓度。


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