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Rat IL-1R2/CD121b ELISA Kit

时间:2016-10-29 12:32来源:未知 作者:admin 点击:
Rat IL-1R2/CD121b ELISA Kit 96T 3500元(货号ER30-96)


【项目简介】
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠IL-1R2抗体,当加入标准品和待测样本时,标准品和待测样本中的大鼠IL-1R2会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗大鼠IL-1R2抗体可与被酶标板捕获的大鼠IL-1R2结合,形成“包被抗大鼠IL-1R2抗体+大鼠IL-1R2+抗大鼠IL-1R2抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠IL-1R2浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠IL-1R2的浓度。


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