【项目简介】
Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide that was originally identified as a CD8+ T cell-derived chemoattractant for CD4+ cells. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. In addition to its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠IL-16抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠IL-16会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠IL-16抗体可与被酶标板捕获的小鼠IL-16结合，形成“包被抗小鼠IL-16抗体+小鼠IL-16+抗小鼠IL-16抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠IL-16浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠IL-16的浓度。