【项目简介】
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠Carbonic Anhydrase VIII抗体，当加入标准品和待测样本时，标准品和待测样本中的小鼠Carbonic Anhydrase VIII会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗小鼠Carbonic Anhydrase VIII抗体可与被酶标板捕获的小鼠Carbonic Anhydrase VIII结合，形成“包被抗小鼠Carbonic Anhydrase VIII抗体+小鼠Carbonic Anhydrase VIII+抗小鼠Carbonic Anhydrase VIII抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠Carbonic Anhydrase VIII浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠Carbonic Anhydrase VIII的浓度。