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Mouse lymphotactin ELISA Kit

时间:2016-10-29 02:30:18来源:本站 作者:admin 点击:335次


【项目简介】
Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant mouse lymphotactin has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠Lymphotactin抗体,当加入标准品和待测样本时,标准品和待测样本中的小鼠Lymphotactin会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗小鼠Lymphotactin抗体可与被酶标板捕获的小鼠Lymphotactin结合,形成“包被抗小鼠Lymphotactin抗体+小鼠Lymphotactin+抗小鼠Lymphotactin抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,小鼠Lymphotactin浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠Lymphotactin的浓度。


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