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Mouse SIGNR1/CD209b ELISA Kit

时间:2016-10-29 02:40:57来源:本站 作者:admin 点击:308次


【项目简介】
SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens. SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR. Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR. The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta  and gamma  isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD. Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%-70% aa sequence identity with mouse DC-SIGN, SIGNR2, 3, and 4, and 64%-68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠SIGNR1抗体,当加入标准品和待测样本时,标准品和待测样本中的小鼠SIGNR1会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗小鼠SIGNR1抗体可与被酶标板捕获的小鼠SIGNR1结合,形成“包被抗小鼠SIGNR1抗体+小鼠SIGNR1+抗小鼠SIGNR1抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,小鼠SIGNR1浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中小鼠SIGNR1的浓度。


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