【项目简介】
Superoxide Dismutases (SODs) are a unique family of metalloproteins that catalyze the dismutation of superoxide anion radicals (O2-) to oxygen (O2) and hydrogen peroxide (H2O2)  O2- + O2- + 2H+ => H2O2 + O2 SOD is ubiquitous in oxygen metabolizing cells protecting these cells against direct and indirect oxygen-mediated free radical damage。Four types of SOD have been defined on the basis of distinctions in their metal cofactors and distribution：Manganese (MnSOD) principally located in the matrix of mitochondria of all aerobes, copper/zinc (Cu/ZnSOD) mainly present in the cytoplasm of eukaryotic cells, iron (FeSOD)，predominantly in the cytosol，chloropasts or mitochondria of prokaryotes as well as extracellular (ECSOD)，which is found in the extracellular fluids or membrane associated in mammals。The properties of Cu/Zn superoxide dismutase are quite different from those of the manganese or iron enzymes。

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人Cu/ZnSOD抗体，当加入标准品和待测样本时，重组或天然的人Cu/ZnSOD会和该抗体结合。洗板后，游离的其他成分被洗去，加入生物素化的抗人Cu/ZnSOD抗体可与被酶标板捕获的人Cu/ZnSOD结合。再次洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的链霉亲和素，生物素和链霉亲和素特异性结合，形成“包被抗人Cu/ZnSOD抗体+人Cu/ZnSOD+生物素化抗人Cu/ZnSOD抗体+链霉亲和素-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人Cu/ZnSOD浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人Cu/ZnSOD的浓度。