【项目简介】
Reg1B also called Lithostatine1β or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted Ctype lectindomaincontaining pancreatic proteins . The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons . 

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人REG1B抗体，当加入标准品和待测样本时，标准品和待测样本中的人REG1B会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人REG1B抗体可与被酶标板捕获的人REG1B结合，形成“包被抗人REG1B抗体+人REG1B+抗人REG1B抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人REG1B浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人REG1B的浓度。