【项目简介】
Lysosomal associated membrane protein 1 (LAMP1), also known as CD107a and LGP120, is an approximately 120 kDa transmembrane glycoprotein that is a major protein component of lysosomal membranes . Mature human LAMP1 consists of a 354 amino acid (aa) intralumenal domain (ECD), a 23 aa transmembrane segment, and a 12 aa cytoplasmic tail . Its lumenal domain is organized into two heavily Nglycosylated regions separated by a Ser/Prorich linker that carries a minor amount of Olinked glycosylation . Within the lumenal domain, human LAMP1 shares approximately 64% aa sequence identity with mouse and rat LAMP1. The sorting of LAMP1 to lysosomes relies on a tyrosine motif in the cytoplasmic tail . In cytotoxic T cells and mast cells, LAMP1 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine . LAMP1 is presented on the plasma membrane during the activation of NK cells, CD8+ T cells, mast cells, basophils, monocytes, and platelets . A glycoform of LAMP1 known as M150 is expressed on the surface of activated macrophages where it promotes T cell costimulation and a Th1 biased immune response . 

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人LAMP-1抗体，当加入标准品和待测样本时，标准品和待测样本中的人LAMP-1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人LAMP-1抗体可与被酶标板捕获的人LAMP-1结合，形成“包被抗人LAMP-1抗体+人LAMP-1+抗人LAMP-1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人LAMP-1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人LAMP-1的浓度。