【项目简介】
Human CD83 is a 40-50 kDa member of the Siglec (or sialicacidbinding immunoglobulinlike lectin) family of transmembrane proteins. CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain . It contains one V type Iglike domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membranebound covalent homodimers, in vivo this does not appear to be the case. In the extracellular region, mouse and human CD83 are 66% aa identical. Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30-35 kDa protein. Human CD83 is active in the mouse system . One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa’s # 20- 52 and # 164-205. In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 . CD83 is a primary marker for dendritic cells. It is also found on B cells , neutrophils , monocytes and macrophages .
 

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CD83抗体，当加入标准品和待测样本时，标准品和待测样本中的人CD83会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人CD83抗体可与被酶标板捕获的人CD83结合，形成“包被抗人CD83抗体+人CD83+抗人CD83抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人CD83浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人CD83的浓度。