【项目简介】
TNF receptor 1 (TNF RI; also called TNF Rp55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A. Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence and 190 aa ECD with a PLAD (preligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteinerich domains), a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain. The ECD of human TNF RI shows 70%, 69%, 80%, 80%, and 73% aa identity with mouse, rat, canine, feline and porcine TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-α trimerbinding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-α. It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria. TNF RI is also a receptor for TNF-β/TNFSF1B (lymphotoxinα).
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人TNFRSF1A抗体，当加入标准品和待测样本时，标准品和待测样本中的人TNFRSF1A会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人TNFRSF1A抗体可与被酶标板捕获的人TNFRSF1A结合，形成“包被抗人TNFRSF1A抗体+人TNFRSF1A+抗人TNFRSF1A抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人TNFRSF1A浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人TNFRSF1A的浓度。