【项目简介】
Carboxypeptidase A2 encoded by the CPA2 gene cleaves the Cterminal amide or ester bond of peptides that have a free Cterminal carboxyl group. It prefers the Cterminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). The purified rhCPA2 corresponds to the pro form, which can be activated and assayed under the conditions described in the Activity Assay Protocol.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CPA2抗体，当加入标准品和待测样本时，标准品和待测样本中的人CPA2会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人CPA2抗体可与被酶标板捕获的人CPA2结合，形成“包被抗人CPA2抗体+人CPA2+抗人CPA2抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人CPA2浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人CPA2的浓度。