【项目简介】
Apolipoprotein A1 (ApoA1) is a 28 kDa glycoprotein that is the major protein component of high density lipoprotein (HDL) particles. HDL particles play a central role in the reverse transport of cholesterol from peripheral tissues to the liver. HDL is known as “good cholesterol” due to its ability to facilitate the removal of cholesterol from macrophage foam cells in atherosclerotic plaques and thereby retard the progression of disease. ApoA1 is secreted from hepatocytes with variable amounts of lipidation. Disc‑like HDL particles increase in size and adopt a spherical shape as they are loaded with additional lipids and cholesterol by ABC family transporters. HDL particle interaction with lipid‑supplying cells is mediated by ApoA1 binding to the scavenger receptor SR‑A1. HDL particles are further modified by the circulating enzymes LCAT (which converts cholesterol to cholesteryl esters), CETP (which transfers cholesteryl esters to LDL/VLDL particles), and PLTP (which transfers phospholipids from HDL to LDL/VLDL particles). ApoA1 is catabolized in the kidney following its binding to Cubulin on renal proximal tubule epithelial cells. Mature human ApoA1 shares 65% and 62% aa sequence identity with mouse and rat ApoA1, respectively.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人ApoA1抗体，当加入标准品和待测样本时，标准品和待测样本中的人ApoA1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人ApoA1抗体可与被酶标板捕获的人ApoA1结合，形成“包被抗人ApoA1抗体+人ApoA1+抗人ApoA1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人ApoA1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人ApoA1的浓度。