【项目简介】
RNase A, also known as ribonuclease A and RNASE1, belongs to ribonucleaseA superfamily. It is a pancreatic-type of secretory ribonuclease. RNaseA is a basic protein and its many positive charges are consistent with its binding to RNA(apoly-anion). Moregenerally, RNaseA is unusually polaror, rather, unusually lacking in hydrophobicgroups, especially aliphatic ones. As a endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclicnucleotides, with a pH optimum near8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and fourtranscript variants encoding the same protein have been identified.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人RNase A抗体，当加入标准品和待测样本时，标准品和待测样本中的人RNase A会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人RNase A抗体可与被酶标板捕获的人RNase A结合，形成“包被抗人RNase A抗体+人RNase A+抗人RNase A抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人RNase A浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人RNase A的浓度。