【项目简介】
CD160 is known to bind to HLA-G1, HLA-C, and HVEM. And upon engagement, it is reported to associate with CD2 in cis under certain conditions. The effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to HLA-G1 initiates apoptosis, and thus impacts angiogenesis. When expressed on CD56DIM NK cells, CD160 signaling in response to HLA-C binding promotes IFN-gamma, TNF-alpha, and IL-6 secretion. And when up‑regulated on CD4+ T cells following activation, CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CD160抗体，当加入标准品和待测样本时，标准品和待测样本中的人CD160会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人CD160抗体可与被酶标板捕获的人CD160结合，形成“包被抗人CD160抗体+人CD160+抗人CD160抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人CD160浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人CD160的浓度。