【项目简介】
Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols. Human SULT1B1 shows 72.3% amino acid sequence identity to mouse SULT1B1.The enzymatic activity of the recombinant human GAL3ST2 is measured using a phosphatase-coupled assay.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人SULT1B1抗体，当加入标准品和待测样本时，标准品和待测样本中的人SULT1B1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人SULT1B1抗体可与被酶标板捕获的人SULT1B1结合，形成“包被抗人SULT1B1抗体+人SULT1B1+抗人SULT1B1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人SULT1B1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人SULT1B1的浓度。