【项目简介】
CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily. Mature human CD7 consists of a 155 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 39 aa cytoplasmic domain. Within the ECD, human CD7 shares 43% and 41% aa sequence identity with mouse and rat CD7, respectively. CD7 is expressed on T cells, NK cells, myeloid progenitor cells, and CD19+ B progenitor cells. Among CD8+ T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells. CD7 associates in cis with CD3 and CD45 and in trans with SECTM. Ligation of CD7 with antibodies or SECTM1 co‑stimulates the activation and proliferation of CD4+ and CD8+T cells (10, 11) and NK cells. It also enhances integrin-mediated adhesion of these cells to Fibronectin, ICAM-1, and VCAM-1. CD7 additionally mediates the Galectin-1 induced apoptosis of T cells and facilitates the fusion of HIV-1 with target cells.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CD7抗体，当加入标准品和待测样本时，标准品和待测样本中的人CD7会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人CD7抗体可与被酶标板捕获的人CD7结合，形成“包被抗人CD7抗体+人CD7+抗人CD7抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人CD7浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人CD7的浓度。