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Human MMP-3 ELISA Kit

时间:2016-10-28 02:14:18来源:本站 作者:admin 点击:327次


【项目简介】
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人MMP-3抗体,当加入标准品和待测样本时,标准品和待测样本中的人MMP-3会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗人MMP-3抗体可与被酶标板捕获的人MMP-3结合,形成“包被抗人MMP-3抗体+人MMP-3+抗人MMP-3抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人MMP-3浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人MMP-3的浓度。


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