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Human CHL-1 ELISA Kit

时间:2016-10-28 04:53:15来源:本站 作者:admin 点击:354次


【项目简介】
Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin typeⅢ domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CHL-1抗体,当加入标准品和待测样本时,标准品和待测样本中的人CHL-1会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗人CHL-1抗体可与被酶标板捕获的人CHL-1结合,形成“包被抗人CHL-1抗体+人CHL-1+抗人CHL-1抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人CHL-1浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人CHL-1的浓度。


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