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Rat IL-8 ELISA Kit

时间:2016-11-01 08:11:40来源:本站 作者:admin 点击:221次

【项目简介】
Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties. CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4, and it can also interact with matrix and cell surface glycosaminoglycans. The bioactivity of CXCL8 is regulated by these truncations, by CXCL8 citrullination at Arg5 (N-terminal to the ELR motif), and by the decoy receptor DARC. CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other. Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation. It triggers the antimicrobial activation of neutrophils through CXCR1. 
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗大鼠IL-8抗体,当加入标准品和待测样本时,重组或天然的大鼠IL-8会和该抗体结合。洗板后,游离的其他成分被洗去,加入生物素化的抗大鼠IL-8抗体可与被酶标板捕获的大鼠IL-8结合。再次洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的链霉亲和素,生物素和链霉亲和素特异性结合,形成“包被抗体+大鼠IL-8+生物素化抗体+链霉亲和素-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,大鼠IL-8浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中大鼠IL-8的浓度。


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