【项目简介】
SLAMfamilymember6,alsoknownasActivatingNKreceptor,NK-T-B-antigen,NTB-A,SLAMF6,KALIandLy108,isasingle-passtypeImembraneproteinwhichbelongstotheCD2subfamilyoftheimmunoglobulinsuperfamily.SLAMF6/Ly108containsoneIg-like(immunoglobulin-like)domain.Itisexpressedbyall(restingandactivated)naturalkillercells(NK),T-andB-lymphocytes.SLAMF6/Ly108triggerscytolyticactivityonlyinnaturalkillercells(NK)expressinghighsurfacedensitiesofnaturalcytotoxicityreceptors.SLAMF6/Ly108isahomodimer.ItinteractswithPTN6and,uponphosphorylation,withPTN11andSH2D1A/SAP.SLAMF6/Ly108undergoestyrosinephosphorylationandassociateswiththeSrchomology2domain-containingprotein(SH2D1A)aswellaswithSH2domain-containingphosphatases(SHPs).ItmayfunctionasacoreceptorintheprocessofNKcellactivation.SLAMF6/Ly108canalsomediateinhibitorysignalsinNKcellsfromX-linkedlymphoproliferativepatients.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人SLAMF6抗体，当加入标准品和待测样本时，标准品和待测样本中的人SLAMF6会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人SLAMF6抗体可与被酶标板捕获的人SLAMF6结合，形成“包被抗人SLAMF6抗体+人SLAMF6+抗人SLAMF6抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人SLAMF6浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人SLAMF6的浓度。