【项目简介】
Acrosomal protein SP-10,also known as Acrosomal vesicle protein 1 and ACRV1, isatestis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and isassociated with the acrosomal membranes and matrix of maturesperm. Regulation of celltype-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1/SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for <1% of the total acrosomal vesicle protein1 message. ACRV1/SP-10 is a testis-specific acrosomal protein that has been detected in several species including humans. It may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人ACRV1抗体，当加入标准品和待测样本时，标准品和待测样本中的人ACRV1会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人ACRV1抗体可与被酶标板捕获的人ACRV1结合，形成“包被抗人ACRV1抗体+人ACRV1+抗人ACRV1抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人ACRV1浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人ACRV1的浓度。