【项目简介】
Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains four EF-hand domains. CALML5/CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. The expression is very low in lung. CALML5/CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor(CAR) is a member of the immunoglobulin(Ig)superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackie virus Bandadeno virus (Ad)infection. CALML5/CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5/CLSP have been characterized by NMR spectroscopy. The mobility of CALML5/CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The over all N-terminal domain be havior is similar both in the full-length protein and in the isolated domain.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人CALML5抗体，当加入标准品和待测样本时，标准品和待测样本中的人CALML5会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人CALML5抗体可与被酶标板捕获的人CALML5结合，形成“包被抗人CALML5抗体+人CALML5+抗人CALML5抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人CALML5浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人CALML5的浓度。