【项目简介】
Reg4 (regenerating isletderived protein 4), also called Reg IV or RELP (Reglike protein), is a 17 kDa secreted glycoprotein belonging to the regenerating gene (Reg) family within the calcium (Ctype) dependent lectin superfamily, although carbohydrate binding of Reg4 is calciumindependent. Human Reg4 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain that contains a Ctype
lectinlike domain (CTLD) and one potential Nlinked glycosylation site. Human Reg4 shares 68% aa sequence identity with mouse and rat, and 77% with canine and porcine Reg4, respectively. Alternate splicing creates
a 134 aa isoform that diverges at aa 56, and a 123 aa isoform that diverges at aa 101. Like other members of the regenerating gene family, Reg4 is preferentially expressed in the gastrointestinal (GI) tract. Reg4 expression is increased within or near inflammation, dysplasia and metaplasia of the GI epithelium, such as inflammatory bowel disease (Crohn’s disease and ulcerative colitis), colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and is often increased in the plasma in these conditions. 

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人REG4抗体，当加入标准品和待测样本时，标准品和待测样本中的人REG4会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人REG4抗体可与被酶标板捕获的人REG4结合，形成“包被抗人REG4抗体+人REG4+抗人REG4抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人REG4浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人REG4的浓度。