【项目简介】
Human acyl-coenzyme A binding domain-containing member6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its Nterminus and two ankyrin motifs at its Cterminus. Inmammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the Nterminus, but they do not share significant homology at the C-terminalregion. ACBD6 is a 32kDa protein that is predicted by sequence analysis to carry an ACBdomain between residues 42 and 125 and two ANK motifs at its Cterminus. This protein bindslong-chainacyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, oversaturated, C16:0-CoA,acylspecies. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人ACBD6抗体，当加入标准品和待测样本时，标准品和待测样本中的人ACBD6会和该抗体结合。洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的的抗人ACBD6抗体可与被酶标板捕获的人ACBD6结合，形成“包被抗人ACBD6抗体+人ACBD6+抗人ACBD6抗体-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，人ACBD6浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人ACBD6的浓度。