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Human Galectin-1 ELISA Kit

时间:2016-10-28 03:23:05来源:本站 作者:admin 点击:266次


【项目简介】
Galectin-1, gene name LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation. Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, integrins alpha 4 beta 1, alpha 5 beta 1 and alpha 4 beta 7, and ganglioside GM1 (2, 3). It is produced in a variety of tissues by cells that include endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells. Most of this expression is cytosolic. Human Galectin-1 shares 88% aa sequence identity with mouse, equine and ovine, 90% with rat, and 87% with bovine and porcine Galectin-1.  

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人Galectin-1抗体,当加入标准品和待测样本时,标准品和待测样本中的人Galectin-1会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗人Galectin-1抗体可与被酶标板捕获的人Galectin-1结合,形成“包被抗人Galectin-1抗体+人Galectin-1+抗人Galectin-1抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人Galectin-1浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人Galectin-1的浓度。


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