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Human Galectin-3 ELISA Kit

时间:2016-10-28 03:24:39来源:本站 作者:admin 点击:242次


【项目简介】
Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain. Mature human Galectin-3 shares 78% and 79% amino acid (aa) sequence identity with mouse and rat Galectin-3, respectively. Human Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted. Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells. Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis. Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation. Galectin-3 can also contribute to chronic inflammation and fibrosis. It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis.

【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗人Galectin-3抗体,当加入标准品和待测样本时,标准品和待测样本中的人Galectin-3会和该抗体结合。洗板后,游离的其他成分被洗去,加入辣根过氧化物酶标记的的抗人Galectin-3抗体可与被酶标板捕获的人Galectin-3结合,形成“包被抗人Galectin-3抗体+人Galectin-3+抗人Galectin-3抗体-HRP”免疫复合物。当加入TMB显色底物后,复合物上连接的HRP催化底物反应,生成蓝色产物,终止反应后变为黄色。在450nm处测定OD值,人Galectin-3浓度与OD450值之间呈正比,可通过绘制标准曲线求出标本中人Galectin-3的浓度。


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