【项目简介】
IL-28 belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons. An active IL-29 gene is absent in mice, and sequence homology between mouse IL-28A and B is 97%. Expression of these cytokines has been observed in a wide variety of cells in response to viral infection and in response to TLR3 or TLR9 ligands.The IFN lambda family signals through a heterodimeric receptor of which one subunit, IL-10R2, is shared with othermembers of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas.Signaling occurs through the Jak/STAT pathway in a similar manner to signaling by the type I IFNs (IFN alpha/beta)and activates many of the same genes despite low sequence homology between the cytokines and receptors in thetwo families. Both IFN families display antiviral, antitumor, and antiproliferative effects, making the IFN lambdaspotential alternatives to IFN-alpha for anticancer and antiviral therapies. Unlike the type I IFNs, which are able tostimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic, epithelial, and sometumor cells. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards theproduction of CD4+CD25+FoxP3+ regulatory T-cells, suggesting a possible immunoregulatory role.
 
【检验原理】
本试剂盒采用双抗体夹心ELISA法。酶标微孔板上预包被有抗小鼠IL-28B抗体，当加入标准品和待测样本时，重组或天然的小鼠IL-28B会和该抗体结合。洗板后，游离的其他成分被洗去，加入生物素化的抗小鼠IL-28B抗体可与被酶标板捕获的小鼠IL-28B结合。再次洗板后，游离的其他成分被洗去，加入辣根过氧化物酶标记的链霉亲和素，生物素和链霉亲和素特异性结合，形成“包被抗小鼠IL-28B抗体+小鼠IL-28B+生物素化抗小鼠IL-28B抗体+链霉亲和素-HRP”免疫复合物。当加入TMB显色底物后，复合物上连接的HRP催化底物反应，生成蓝色产物，终止反应后变为黄色。在450nm处测定OD值，小鼠IL-28B浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中小鼠IL-28B的浓度。